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OriGene
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Image Search Results
Journal: Oncotarget
Article Title: SETD6 regulates NF-κB signaling in urothelial cell survival: Implications for bladder cancer
doi: 10.18632/oncotarget.14750
Figure Lengend Snippet: ( A ) Validation of relative SETD6 expression in different bladder cells was carried out using qPCR. p ≤ 0.05 were considered to be statistically significant (*). The samples were measured in triplicates and the experiment was repeated 3 times. ( B ) cDNA array was performed with bladder cancer tissues ( n = 24) representing different stages and SETD6 expression was analyzed by qPCR. ( C ) Basal level of SETD6 protein was detected using anti-SETD6 antibody by western blotting. ( D ) Immunocytochemistry in different bladder cancer cell lines cells showing cytoplasmic and nuclear SETD6. ( E ) SETD6 protein was detected using anti-SETD6 antibody by western blot in bladder cancer tissues ( n = 9) compared to non-cancerous bladder tissues.
Article Snippet:
Techniques: Biomarker Discovery, Expressing, Western Blot, Immunocytochemistry
Journal: Clinical Epigenetics
Article Title: Feasibility of quantifying SDC2 methylation in stool DNA for early detection of colorectal cancer
doi: 10.1186/s13148-017-0426-3
Figure Lengend Snippet: Schematic diagram and analytic performance of me SDC2 LTE-qMSP. a Outline of me SDC2 LTE-qMSP. LTE was first performed using two primers: SDC2 and COL2A1 antisense primers attached to a universal primer to enrich methylated SDC2 DNA, and consecutive duplex real-time PCR was carried out using methylation-specific SDC2 sense primer, COL2A1 -specific sense primer, universal primer, and probes. b The LoD of me SDC2 LTE-qMSP was compared with that of me SDC2 -qMSP. Different amounts of HCT116 genomic DNA were diluted in unmethylated genomic DNA in total 20 ng of genomic DNA
Article Snippet: The unmethylated genomic DNA was prepared by whole genome amplification of
Techniques: Methylation, Real-time Polymerase Chain Reaction